We studied the interplay between the aggregation and protonation behavior of 2.5 kDa linear PEI by pH probing, vapor pressure osmometry, dynamic light scattering, and ninhydrin assay.
Malvern zetasizer wavelength free#
As a result, in solution PEI exhibits multiple buffering mechanisms, and polyelectrolyte states that shift between aggregated and free forms. The polymer has closely-spaced amines with weak-base protonation capacity, and a hydrophobic backbone that is kept unaggregated by intra-chain repulsion.
Malvern zetasizer wavelength software#
Research software option to give access to further features and analysis algorithms for the light scattering specialist.21CFR part 11 software option to enable compliance with ER/ES.A ‘Quality Factor’ and ‘Expert Advice System’ gives the confidence of having an expert at your shoulder.Optional protein calculators, including protein charge, A2, kD, and molecular shape.Sample concentrations from 0.1ppm to 40%w/v.
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Protein size measurement as dilute as 0.1mg/mL (Lysozyme).Microrheology option to measure viscosity and viscoelasticity.
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Size measurement from 0.3nm (diameter) to 10 microns using patented NIBS (Non-Invasive Back Scatter) technology.Particle and molecule size, translational diffusion, electrophoretic mobility, zeta potential of particles at high and low concentrations, viscosity and viscoelasticity of protein and polymer solutions, concentration, MW, A2, kD.Īn optional accessory enables measurement of the zeta potential of solid surfaces. The flow mode option enables the system to be connected to an SEC or an FFF system to use as a detector for the size of proteins or nanoparticles.Ī choice of cuvettes are available, from disposable single-use to specific cells for viscous or high concentration samples or measuring the zeta potential of surfaces. In addition, a microrheology option is available for measuring sample viscosity and viscoelastic properties, as well as a Protein Measurement option for protein mobility measurements. Using Non-Invasive Backscatter optics (NIBS) it has significantly better performance than systems using 90 degree scattering optics. The ZSP also incorporates a zeta potential analyzer that uses electrophoretic light scattering for particles, molecules and surfaces, and a molecular weight analyzer using static light scattering. The Georgia Tech Zetasizer Nano ZS, located in the Georgia Tech Biocleanroom, is a high performance two angle particle and molecular size analyzer for the enhanced detection of aggregates and measurement of small or dilute samples, and samples at very low or high concentration using dynamic light scattering with ‘NIBS’ optics.